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    Low-fouling properties in serum of carboxylic-oligo(ethylene glycol)-based interfaces

    Year: 2021

    Journal: Colloid Surf. A-Physicochem. Eng. Asp., Volume 618, JUN 5

    Authors: Santos, Adriano; Garcia Nicholson, Melany Isabel; Feliciano, Gustavo Troiano; Bueno, Paulo Roberto

    Organizations: CNPq; CAPES [88887.463447/2019-00]; FAPESP [2017/24839-0]

    Keywords: Low-fouling interfaces; QCM-D; Molecular dynamics; Protein adsorption; Self-assembled monolayers

    In the present work we evaluated the low-fouling feature of a carboxylic-oligo(ethylene glycol) self-assembled monolayer (carboxylic-OEG-SAM) interface using a quartz crystal microbalance with dissipation monitoring (QCM-D). QCM-D measurements allowed us to estimate the amount of protein loading in two different serum dilutions at two different interfaces: bare gold and carboxylic-OEG. The observed amount of protein adsorbed onto bare gold is about twice higher that of carboxylic OEG interface, confirming the low-fouling characteristics of OEG-modified surfaces. Additionally, QCM-D results demonstrated the existence of two protein adsorption regimes, a faster and a slower, with distinct dissipation energies which was modelled by a two-step kinetic model. The faster regime was attributed to the adsorption of proteins into free sites of the carboxylic-OEG-SAM in a rigid binding process, followed by a slower and more viscoelastic adsorption process ascribed to structural conformational changes; this slower step conforms with the filling of remaining free sites associated with the steric hindrance in which protein-protein interactions defines the slower rate constant for the adsorption.
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