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    Monitoring the surface tension by the pendant drop technique for detection of insulin fibrillogenesis

    Year: 2021

    Journal: Anal. Methods, Volume 13, SEP 28, page 4174–4178

    Authors: Siposova, Katarina; Sedlakova, Dagmar; Musatov, Andrey

    Organizations: Slovak Grant Agency VEGA [2/0094/21]; MVTS SK-TW Amazon [SAS-MOST JRP 2017/6]; NATO Science for Peace and Security Programme [G5683]

    Monitoring the aggregation of amyloid-prone proteins is critical for understanding the mechanism of amyloid fibril formation. Insulin, when dissolved in low pH buffer, has a surface tension of 61-64 mN m(-1), as measured by the pendant drop technique. Formation of insulin amyloid fibrils resulted in the increase of the surface tension values up to 71.2-73.5 mN m(-1). The kinetics of fibril formation and fibril morphology were validated by ThT fluorescence and AFM, respectively. The results demonstrate that monitoring the surface tension by the pendant drop technique is a valuable tool for the detection of insulin amyloid aggregation.
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