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    Nano-hydroxyapatite incorporated quince seed mucilage bioscaffolds for osteogenic differentiation of human adipose-derived mesenchymal stem cells

    Year: 2022

    Journal: Int. J. Biol. Macromol., Volume 195, JAN 15, page 492–505

    Authors: Genc, Cigdem Cetin; Yilmaz, Hilal Deniz; Karaca, Burak; Kiran, Fadime; Arslan, Yavuz Emre

    Organizations: Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit [TSA-2019-2933]

    Keywords: Quince seed mucilage; Nano-hydroxyapatite; Bioscaffold; Osteoinductive; Non-load bearing bone grafts; Bone tissue engineering

    In this study, the therapeutic hydrocolloid quince seed mucilage (QSM) from Cydonia oblonga Miller fruit is enriched with needle-like nano-hydroxyapatite (nHAp) crystals to fabricate a novel biomimetic osteogenic bioscaffold. The molecular weight (M-w) of water-based extracted QSM was measured with GPC (8.67 x 10(5) g/ mol), and the composite blend was prepared at a ratio of 1:1 (w/w) QSM(aq) and nHAp. The porous bioscaffolds were manufactured by the freeze-drying method, and evaluated in-depth with advanced analyses. The XRD, ATR-FTIR, SEM-EDX, and elemental mapping analyses revealed a uniform coated semi-crystalline structure with no covalent bindings between QSM and nHAp. Moreover, due to the hydrocolloid backbone, a supreme swelling ratio (w/w, 6523 +/- 190%) with suitable pore size (208.12 +/- 99.22 mu m) for osteogenic development was obtained. Further, the cytocompatible bioscaffolds were evaluated for osteogenic differentiation in vitro using human adipose-derived mesenchymal stem cells (hAMSCs). The immuno/histochemical (I/HC) staining revealed that the cells with the spherical morphology invaded the pores of the prepared bioscaffolds. Also, relatively early up-regulated osteogenic markers were observed by the qRT-PCR analyses. Overall, it is believed that the QSM-nHAp bioscaffolds might be favorable in non-load bearing applications, especially in the cranio-maxillofacial region, due to their regenerative, bendable, and durable features.
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