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    Newly-designed collagen/polyurethane bioartificial blend as coating on bioactive glass-ceramics for bone tissue engineering applications

    Year: 2019

    Journal: Mater. Sci. Eng. C-Mater. Biol. Appl., Volume 96, MAR, page 218–233

    Authors: Caddeo, Silvia; Mattioli-Belmonte, Monica; Cassino, Claudio; Barbani, Niccoletta; Dicarlo, Manuela; Gentile, Piergiorgio; Baino, Francesco; Sartori, Susanna; Vitale-Brovarone, Chiara; Ciardelli, Gianluca

    Organizations: Italian Ministry of Education, University and Research (MIUR) under the PRIN 2010-2011 programmme [2010J8RYS7]

    Keywords: Bioartificial blend; Bioactive glass; Bone tissue engineering; Collagen; Functionalisation; Polyurethanes

    In the present work, a new combination of synthetic and natural biomaterials is proposed for bone tissue engineering (BTE) applications. In order to mimic the inorganic and organic phases of bone extracellular matrix (ECM), a bioactive glass-ceramic deriving from a SiO2-P2O5-CaO-MgO-Na2O-K2O parent glass, acting as a substrate in form of a slice, was surface-functionalised with a type I collagen-based coating. In particular, the collagen was blended with a water soluble polyurethane (PUR), synthesised from poly(ethylene glycol), 1,6-hexamethylene diisocyanate and N-BOC-serinol. The PUR was designed to expose amino groups on the polymeric chain, which can be exploited for the blend stabilisation through crosslinking. The newly synthesised PUR demonstrated to be non-cytotoxic, as assessed by a biological test with MG-63 osteoblast-like cells. The collagen/PUR blend showed good biocompatibility as well. The polymeric coating on the glass-ceramic samples was produced by surface-silanisation, followed by further chemical grafting of the blend, using genipin as a cross linker. The glass-ceramic surface was characterised at each functionalisation step, demonstrating that the procedure allowed obtaining a covalent link between the blend and the substrate. Finally, biological tests performed using human periosteal derived precursor cells demonstrated that the proposed polymer-coated material was a good substrate for bone cell adhesion and growth, and a good candidate to mimic the composite nature of the bone ECM.
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