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    One-Step Approach to Prepare Transparent Conductive Regenerated Silk Fibroin/PEDOT:PSS Films for Electroactive Cell Culture

    Year: 2022

    Journal: ACS Appl. Mater. Interfaces, Volume 14, JAN, page 123–137

    Authors: Zhuang, Ao; Huang, Xiangyu; Fan, Suna; Yao, Xiang; Zhu, Bo; Zhang, Yaopeng

    Organizations: National Natural Science Foundation of China [52173031, 51903045, 51703033]; Program of Shanghai Academic/Technology Research Leader [20XD1400100]; Natural Science Foundation of Shanghai [20ZR1402400]; Basic Research Project of the Science and Technology Commission of Shanghai Municipality [21JC1400100]; Fundamental Research Funds for the Central Universities [2232020D-04, 2232019A3-06, 2232019D302]; National Key Research and Development Program of China [2016YFA0201702, 2018YFC1105800]; International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality [19520744500]

    Keywords: regenerated silk fibroin; PEDOT:PSS; transparent conductive film; electroactive biomaterial; electrical stimulation

    Silk fibroin (SF)-based electroactive biomaterials with favorable electroconductive property and transparency have great potential applications for cell culture and tissue engineering. Poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PE-DOT:PSS) is an excellent candidate as a conductive component, which has been widely used in the field of bioelectronics; however, it is hard to be directly coated onto the surface of regenerated SF (RSF) materials with good stability under a cell culture environment. In this study, a one-step facile PEDOT:PSS modification approach for RSF films based on a suitable post-treatment process of RSF was developed. PEDOT:PSS was successfully embedded and fixed into the shallow surface of an RSF film, forming a tightly conjunct conductive layer on the film surface based on the conformation transition of RSF during the post-treatment process. The conductive layer demonstrated a PSS-rich surface and a PEDOT-rich bulk structure and showed excellent stability under a cell culture environment. More specifically, the robust RSF/PEDOT:PSS film achieved in the post-treatment formula with 70% ethanol proportion possessed best comprehensive properties such as a sheet resistance of 3.833 x 10(3) Omega/square, a conductivity of 1.003 S/cm, and transmittance over 80% at maximum in the visible range. This kind of electroactive biomaterial also showed good electrochemical stability and degradable properties. Moreover, pheochromocytoma-derived cell line (PC12) cells were cultured on the RSF/PEDOT:PSS film, and an effective electrical stimulation cell response was demonstrated. The facile preparation strategy and the good electroconductive property and transparency make this RSF/PEDOT:PSS film an ideal candidate for neuronal tissue engineering and further for biomedical applications.
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