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    Photoclick chemistry to create dextran-based nucleic acid microarrays

    Year: 2019

    Journal: Anal. Bioanal. Chem., Volume 411, OCT, page 6745–6754

    Authors: Diaz-Betancor, Zeneida; Banuls, Maria-Jose; Maquieira, Angel

    Organizations: MINECO [BIHOLOG CTQ/2016/75749-R]

    Keywords: Fluorescence microarray; Hydrogel; Dextran; Thiol-acrylate click chemistry; Nucleic acids

    In the literature, there are reports of the utilization of various hydrogels to create generic platforms for protein microarray applications. Here, a novel strategy was developed to obtain high-performance microarrays. In it, a dextran hydrogel is used to covalently immobilize oligonucleotides and proteins. This method employs aqueous solutions of dextran methacrylate (Dx-MA), which is a biocompatible photopolymerizable monomer. Capture probes are immobilized inside the hydrogel via a light-induced thiol-acrylate coupling reaction at the same time as the dextran polymer is formed. Hydrogel microarrays based on this technique were prepared on different surfaces, such as a Blu-ray Disk and polycarbonate or alkene-functionalized glass slides, and these systems showed high probe-loading capabilities and good biorecognition yields. This methodology presents advantages such as a low cost, a short analysis time, a low limit of detection, and multiplexing capabilities, among others. Confocal fluorescence microscopy analysis demonstrated that in these hydrogel-based microarrays, receptor immobilization and the biorecognition event occurred within the hydrogel and not merely on the surface.
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