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    Polystyrene-block-polyethylene oxide thin films: In vitro cytocompatibility and protein adsorption testing

    Year: 2020

    Journal: Biointerphases, Volume 15, JAN

    Authors: Monteiro, Isabela A.; Kollmetz, Tarek; Musson, David S.; McGlashan, Sue R.; Malmstrom, Jenny

    Organizations: Marsden Fund CouncilRoyal Society of New ZealandMarsden Fund (NZ); Rutherford Discovery Fellowship; University of Auckland

    Polystyrene-block-polyethylene oxide (PS-b-PEO) coated surfaces have been explored as cell culture substrates in the past decade. However, their cytocompatibility has not been extensively assessed. In this study, the in vitro cytocompatibility of PS-b-PEO was investigated. Cellular morphology, metabolic activity, and viability were evaluated at 1, 3, and 5 days after cell seeding. Viability was greater than 90% throughout the 5 days culture, with abundant cell spreading evident by the formation of prominent F-actin stress fibres. The cytocompatibility study was complemented by the analysis of adsorption of a range of extracellular matrix proteins on PS-b-PEO thin films by quartz crystal microbalance with dissipation. Protein adsorption tests revealed that there was no significant difference in protein adhesion between surfaces with a PEO domain coverage of approximate to 28%, compared to the homogeneous polystyrene control. The findings demonstrate that PS-b-PEO thin films are cytocompatible and are a favourable surface coating for cell culture studies.
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