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    Polyunsaturated Phospholipid Modified Membrane Degradation Catalyzed by a Secreted Phospholipase A2

    Year: 2019

    Journal: Langmuir, Volume 35, SEP 10, page 11643–11650

    Authors: Zhang, Pin; Villanueva, Veronica; Kalkowski, Joseph; Liu, Chang; Pham, Tiep; Perez-Salas, Ursula; Bu, Wei; Lin, Binhua; Liu, Ying

    Organizations: National Science Foundation-Nanomanufacturing Program (NSF CAREER) [1350731]; Division of Chemistry (CHE), National Science Foundation [NSF/CHE-1834750]; Division of Materials Research (DMR), National Science FoundationNational Science Foundation (NSF) [NSF/CHE-1834750]; U.S. DOEUnited States Department of Energy (DOE) [DE-AC02-06CH11357]

    To optimize the compositions of the lipid-based nanomedicine and to advance understanding of the roles of polyunsaturated phospholipids in biological membranes, this study examined the effects of polyunsaturated phospholipids on the degradation of giant unilamellar vesicles catalyzed by a secreted phospholipase A2 (sPLA(2)) using fluorescence microscopy. Molecular interfacial packing, interaction, and degradation of the films containing various mixing ratios of saturated and polyunsaturated phospholipids were quantified using a Langmuir trough integrated with synchrotron X-ray surface scattering techniques. It was found that a high molar fraction (0.63 and above) of polyunsaturated phospholipids not only enhanced the rate of sPLA(2)-catalyzed vesicle degradation but also changed the vesicle deformation process and degradation product morphology. Hydrolysis of the saturated phospholipids generated highly ordered liquid crystal domains, which was reduced or prohibited by the presence of the polyunsaturated phospholipids in the reactant film.
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