Keywords: TGF-beta; polyurethanes; HSNGLPL peptide; inflammation; ER stress
The synthesized short peptide-polymers would provide key functions for tissue regeneration and repair, through enriching bioactive molecules on polymers or releasing these molecules pre-conjugatedon the materials. We have developed a degradable polyurethane (PU) bearing HSNGLPL peptide, which has affinity binding ability to transforming growth factor-betas (TGF-beta). For deeply understanding spatial release of TGF-beta from the PU polymers and its localized bioactivity, quartz crystal microbalance (QCM) and Elisa test were used to verify TGF-beta binding capacities in vitro and in vivo. The PU polymers, with or without pre-conjugating of TGF-beta were implanted into gastronomies muscle (GN) of C57BL/6 mice, for addressing TGF-beta release from the polymers and its bio-regulating function in vivo. QCM result shows that PU bearing HSNGLPL peptide has affinity binding ability to TGF-beta in vitro. Intramuscular implanting experiment further supports the enrichment efficiency of TGF-beta on PU polymers in vivo. The detecting data involving intramuscular inflammatory infiltration triggered by the implants, myofiber regeneration, muscular fibrosis degree, and activation of endoplasmic reticulum stress (ER stress), evidence TGF-beta can be released from PU polymers, and exerts regulating effects on the material-induced inflammation. Thus, our present results suggest it is feasible to improve biocompatibility of PU polymers in vivo, by pre-bearing bioactive molecules on materials before the implanting. (C) 2017 Wiley Periodicals, Inc.
