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    Preparation and characterization of 3D human glioblastoma spheroids using an N-octanoyl glycol chitosan hydrogel

    Year: 2021

    Journal: Int. J. Biol. Macromol., Volume 185, AUG 31, page 87–97

    Authors: Bae, Yoonhee; Joo, Chanyang; Park, Kyoung Hwan; Kang, Sun-Woong; Huh, Kang Moo; Choi, Joon Sig

    Organizations: Bio&Medical Technology Development Program [NRF-2019M3A9H1103331]; Ministry of Science [NRF-2020R1A2C2100794]; Ministry of Education - Korean government [NRF-2019R1I1A1A01061429, NRF-2020R1I1A3070280]

    Keywords: 3D culture; Spheroid; N-octanoyl glycol chitosan; Ultra-low adhesion; Tumor model

    The current 2D culture model systems developed for drug screening are not sufficient to reflect the characteristics of in vivo solid tumors. Therefore, more effective in vitro tumor model systems must be developed for translational studies on therapeutic drug screening and testing. Herein, we report a new ultra-low adhesion (ULA) hydrogel for generating 3D cancer cell spheroids as tumor models in vitro. N-octanoyl glycol chitosan (OGC) was synthesized and coated onto the surface of a typical cell culture dish. Cell spheroids were effectively formed on the OGCcoated surface, and phenotypes of the tumor cells were well maintained during culture. More importantly, U373-MG cells cultured on OGC-coated plates were more resistant to doxorubicin than cells cultured on typical plates. Our OGC-based ULA system may offer a convenient method for 3D cell culture to provide enhanced performance in cancer research, drug screening and toxicology.
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