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    Preparation of a beta-tricalcium phosphate nanocoating and its protein adsorption behaviour by quartz crystal microbalance with dissipation technique

    Year: 2018

    Journal: Colloid Surf. B-Biointerfaces, Volume 162, FEB 1, page 1–7

    Authors: Pang, Dongwen; He, Liyong; Wei, Luxi; Zheng, Huade; Deng, Chunlin

    Keywords: Beta-tricalcium phosphate; Electrophoretic deposition; Bovine serum albumin; Lysozyme; Adsorption

    Beta-tricalcium phosphate (beta-TCP) nanocoatings, which can be analysed using a quartz crystal microbalance with dissipation technique (QCM-D), were fabricated on a gold surface by electrophoretic deposition. The influences of electric field intensity and electrophoresis time were investigated. The adsorption behaviours of bovine serum albumin (BSA) and lysozyme (LSZ) on Au and beta-TCP surfaces were observed in real time by QCM-D. The homogeneous beta-TCP nanocoating with moderately sized particles on gold surface was fabricated at 25 V/cm for 5 min, and it met the requirements for the QCM-D experiment. The adsorption behaviour of BSA was different from that of LSZ, which was caused by the differences of protein properties. The adsorption quantity of BSA on a beta-TCP surface was higher than that on a gold surface. However, the adsorption amount of LSZ on a beta-TCP surface was lower than that on a gold surface. The electrostatic force was the major factor affecting the adsorption quantities of BSA and LSZ on Au and beta-TCP surfaces based on the investigation of various factors. The findings reported here will be useful for understanding the mechanism of the interaction between biomaterials and proteins. (C) 2017 Elsevier B.V. All rights reserved.
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