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    Production, characterization, evaluation and toxicity assessment of a Bacillus cereus UCP 1615 biosurfactant for marine oil spills bioremediation

    Year: 2020

    Journal: Mar. Pollut. Bull., Volume 157, AUG

    Authors: Durval, Italo Jose B.; Mendonca, Ana Helena R.; Rocha, Igor, V; Luna, Juliana M.; Rufino, Raquel D.; Converti, A.; Sarubbo, L. A.

    Organizations: Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ); Programa de Pesquisa & Desenvolvimento da Agencia Nacional de Energia Eletrica (ANEEL); Termocabo S.A., Brazil; Centrais Eletricas da Paraiba (EPASA); Centrais Eletricas de Pernambuco S.A. (EPESA)

    Keywords: Bacillus cereus; Biosurfactant; Toxicity; Poecilia vivipara; Anomalocardia brasiliana; Bioremediation

    In this study, Bacillus cereus was cultivated in a mineral medium composed of 2% frying oil and 0.12% peptone to produce a biosurfactant. The production was scaled up from flasks to 1.2-, 3.0- and 50-L bioreactors, where surface tension achieved 28.7, 27.5 and 32 mN/m and biosurfactant concentration 4.3, 4.6 and 4.7 g/L, respectively. The biosurfactant was characterized as anionic, while nuclear magnetic resonance, thin-layer chromatography and gas chromatography analyses revealed its lipopeptide nature. Toxicity tests showed survival rates of the fish Poecilia vivipara and the bivalve Anomalocardia brasiliana higher than 90% and 55%, respectively, thus suggesting the use of this biosurfactant in marine environment depollution. Moreover, the biosurfactant stimulated the growth of autochthonous microorganisms independently of the presence of motor oil in bioassays performed in seawater. These results demonstrate that the biosurfactant is biocompatible and has potential for industrial-scale production and application to bioremediation of oil spills-polluted marine environment.
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