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    Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation

    Year: 2020

    Journal: Int. J. Pharm., Volume 574, JAN 25

    Authors: Von Borowski, Rafael Gomes; Barros, Muriel Primon; da Silva, Denise Brentan; Lopes, Norberto Peporine; Zimmer, Karine Rigon; Staats, Charley Christian; de Oliveira, Cristiane Bernardes; Giudice, Emmanuel; Gillet, Reynald; Macedo, Alexandre Jose; Baggio Gnoatto, Simone Cristina; Zimmer, Aline Rigon

    Organizations: Conselho Nacional de Desenvolvimento Cientifico e Tecnologico -CNPq, BrazilConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [312351/2017-8, 303353/2016-3]; Fundacao de Amparo a Pesquisa do Rio Grande do Sul FAPERGS, BrazilFundacao de Amparo a Ciencia e Tecnologia do Estado do Rio Grande do Sul (FAPERGS) [PRONEM 16/2551-0000244-4]; CAPES-COFECUB, BrazilCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [88887.130212/2017-01]; Instituto Nacional de Ciencia e Tecnologia para Inovacao Farmaceutica -INCT-if, Brazil; Graduate Program of Pharmaceutical Sciences -PPGCF/UFRGS, Brazil; Fundacao de Amparo a Pesquisa do Estado de Sao Paulo -FAPESP, BrazilFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2009/54098-6, 2012/18031]

    Keywords: Biofilm; Antibiofilm; Antiadhesive; Capsicum; Peptide; Coating

    Medical devices (indwelling) have greatly improved healthcare. Nevertheless, infections related to the use of these apparatuses continue to be a major clinical concern. Biofilms form on surfaces after bacterial adhesion, and they function as bacterial reservoirs and as resistance and tolerance factors against antibiotics and the host immune response. Technological strategies to control biofilms and bacterial adhesion, such as the use of surface coatings, are being explored more frequently, and natural peptides may promote their development. In this study, we purified and identified antibiofilm peptides from Capsicum baccatum (red pepper) using chromatography-tandem mass spectrometry, MALDI-MS, MS/MS and bioinformatics. These peptides strongly controlled biofilm formation by Staphylococcus epidermidis, the most prevalent pathogen in device-related infections, without any antibiotic activity. Furthermore, natural peptide-coated surfaces dislayed effective antiadhesive proprieties and showed no cytotoxic effects against different representative human cell lines. Finally, we determined the lead peptide predicted by Mascot and identified CSP37, which may be useful as a prime structure for the design of new antibiofilm agents. Together, these results shed light on natural Capsicum peptides as a possible antiadhesive coat to prevent medical device colonization.
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