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    Round Robin test on V-shape bio-imaging transfer standard for determination of the Instrument Transfer Function of 3D optical profilers

    Year: 2018

    Authors: Bermudez, Carlos; Artigas, Roger; Martinez, Pol; Nolvi, Anton; Jarvinen, Miikka; Hggstrom, Edward; Kassamakov, Ivan

    Country: San Francisco, CA

    Keywords: Bio transfer standard; Optical Profiler; Coherence Scanning Interferometry; Instrument Transfer Function

    A V-shape Bio-Transfer-Standard (V-BTS), developed and produced at the University of Helsinki (UH), was measured in two laboratories. In comparison to Siemens Star calibration specimens, the V-BTS performs better at high lateral frequencies close to the diffraction limit of the optical instrument. This peunits deteunining of the Instrument Transfer Function (ITF). The V-BTS features two lipid bilayer steps that partly overlap each other at an angle of 20, with an average height of 4.6 +/- 0.1 nm. The Round Robin (RR) test aims to determine whether the V-BTS and the developed application protocol work with different optical profilers in different laboratories. First the artefact was measured at Sensofar-Tech, S.L. using an S-neox profiler working in Phase Shifting Interferometry mode. Then V-BTS was measured at UH using a custom-built Scanning White Light Interferometer. All measurements done by four different operators at the two laboratories have a range or standard deviation of 0.1 nm which agrees with the theoretical estimates and with measurements done using an atomic force microscope and with a surface plasmon resonance based instrument. The RR results show the applicability of the V-BTS for calibration and for ITF characterization of 3D optical profilers.
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