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    Sequential Inkjet Printing of Lysozyme and Tyrosinase on Polyamide Fabric: Sustainable Enzyme Binding on Textile Surface

    Year: 2022

    Journal: Adv. Mater. Interfaces, Volume 9, AUG

    Authors: Biswas, Tuser T.; Yu, Junchun; Nierstrasz, Vincent A.

    Organizations: University of Boras; TEKO (Sveriges Textil- och Modeforetag); Sparbanksstiftelsen Sjuharad

    Keywords: antimicrobial activity; antibacterial effect; digital printing; nylon; plasma; polyphenol oxidase

    An ink containing tyrosinase catalyzes the tyrosine residues on lysozyme protein to bind it on a plasma-treated polyamide-6,6 (PA) fabric. Inkjet printing enables controlled and sequential deposition of two enzymes on PA which is necessary for proper binding. The effect of different printing sequences on crosslinking stability and enzymatic activity is presented. The lysozyme bound on the fabric shows satisfactory antimicrobial activity. The printed fabric retains about 68% of the ink activity when tyrosinase is printed before lysozyme. Further, this fabric retains about 24% of the initial activity up to four reuses. The fabric shows acceptable inhibition of bacterial growth and retains almost half of its initial activity when cold stored for a month. This work shows the potential of protein binding on textile surface using various means of sustainable technologies, namely enzyme, inkjet, and plasma.
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