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    Study of the interactions of gold nanoparticles functionalized with aminolevulinic acid in membrane models

    Year: 2021

    Journal: Colloid Surf. B-Biointerfaces, Volume 205, SEP

    Authors: da Silva, Rafael Leonardo C. G.; Goncalves, Karina de Oliveira; Courrol, Lilia Coronato; Caseli, Luciano

    Organizations: Fundacao de Amparo a` Pesquisa do Estado de Sao Paulo (FAPESP) [2018/00291-9, 2019/03239-0, 2018/22214-6, 2017/23686-6]

    Keywords: Gold nanoparticles; Drug delivery; Tensiometry; PM-IRRAS; Langmuir monolayers

    Gold nanoparticles have been intensively studied in cancer therapy to improve drug release, increasing thera-peutic action and reducing adverse effects. The interaction between gold nanoparticles and cell membranes can give information about the cell internalization. In this study, gold nanoparticles with aminolevulinic acid (5-ALA) were synthesized using the photoreduction method (5-ALA: AuNPs). The prodrug 5-ALA is responsible for protoporphyrin IX synthesis inside the cell and allows the use of therapies as photodynamic and sonodynamic therapies. The cytotoxicity test was performed on a breast cancer tumor line (MCF-7), and high Content Screening assay was applied to evaluate the entry of nanoparticles into cells. DPPS Langmuir monolayers were assembled at the air/water interface and employed as a simplified membrane model for half of a tumorigenic cell membrane. We assessed the molecular interactions between 5-ALA: AuNPs and phospholipids using tensiometry (pi-A isotherms) and vibrational spectroscopy (PM-IRRAS) experiments. We found that the functionalized gold nanoparticles strongly interact with DPPS polar head groups (especially phosphate and carbonyl), changing the phospholipid hydration and leading to a general decrease in the monolayer conformational order. This work then probes that specific interaction between 5-ALA: AuNPs and the negatively charged phospholipid can be assessed using Langmuir monolayers as simplified biomembrane models.
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