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    Surface-Bound Antibiotic for the Detection of beta-Lactamases

    Year: 2019

    Journal: ACS Appl. Mater. Interfaces, Volume 11, SEP 11, page 32599–32604

    Authors: Miller, Lisa M.; Silver, Callum D.; Herman, Reyme; Duhme-Klair, Anne-Kathrin; Thomas, Gavin H.; Krauss, Thomas F.; Johnson, Steven D.

    Keywords: antibiotics; antimicrobial resistance; beta-lactamases; surface chemistry; surface-sensitive biosensor

    Antimicrobial resistance (AMR) has been identified as a major threat to public health worldwide. To ensure appropriate use of existing antibiotics, rapid and reliable tests of AMR are necessary. One of the most common and clinically important forms of bacterial resistance is to beta-lactam antibiotics (e.g., penicillin). This resistance is often caused by beta-lactamases, which hydrolyze beta-lactam drugs, rendering them ineffective. Current methods for detecting these enzymes require either time-consuming growth assays or antibiotic mimics such as nitrocefin. Here, we report the development of a surface-bound, clinically relevant beta-lactam drug that can be used to detect beta-lactamases and that is compatible with a range of high-sensitivity, low-cost, and label-free analytical techniques currently being developed for point-of-care-diagnostics. Furthermore, we demonstrate the use of these functionalized surfaces to selectively detect beta-lactamases in complex biological media, such as urine.
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