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    Surface mediated non-viral gene transfection on titanium substrates using polymer electrolyte and nanostructured silicate substituted calcium phosphate pDNA (NanoSiCaPs) composites

    Year: 2018

    Journal: Mater. Today Commun., Volume 16, SEP, page 169–173

    Authors: Shekhar, Sudhanshu; Lee, Boeun; Roy, Abhijit; Candiello, Joe; Kumta, Prashant N.

    Organizations: NSF [CBET-0933153]; NSF-ERC [EEC-0812348]; Edward R. Weidlein Chair Professorship Funds; Center for Complex Engineered Multifunctional Materials (CCEMM), Swanson School of Engineering; University of Pittsburgh

    Keywords: Calcium phosphate (CaP); Nanoparticles; Gene delivery; Plasmid DNA; Hydroxyapatite; Bioceramics

    Exploiting gene delivery from the surfaces of bio-functionalized implants is a unique strategy to facilitate tissue regeneration and integration. However, it has been challenging, due to the difficulty for incorporation and delivey of sufficient payloads of plasmid DNA (pDNA) to cells from the implant surface allowing efficient gene expression. Herein, we describe a novel and simple coating approach using nanostructured silicate substituted calcium phosphate pDNA complexes termed as NanoSiCaPs, that preferentially adsorb onto titanium (Ti) substrates coated with poly (diallyldimethylammonium chloride) (PDADMAC). The Ti-polyelectrolyte NanoSiCaPs assemblies aptly called PNA, deliver pDNA to MC3T3-E1 cells (pre-osteoblast cell line), induce protein production, without eliciting any cytotoxicity, while simultaneously encouraging cell attachment on the substrate.
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