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    Surface Triggered Self-Assembly of Fmoc-Tripeptide as an Antibacterial Coating

    Year: 2020

    Journal: Front. Bioeng. Biotechnol., Volume 8, AUG 7

    Authors: Criado-Gonzalez, Miryam; Iqbal, Muhammad Haseeb; Carvalho, Alain; Schmutz, Marc; Jierry, Loic; Schaaf, Pierre; Boulmedais, Fouzia

    Keywords: peptides; hydrogels; antimicrobial; enzyme-assisted self-assembly; nanoparticles

    In western countries, one patient on twenty will develop a nosocomial infection during his hospitalization at health care facilities. Classical antibiotics being less and less effective, this phenomenon is expanding year after year. Prevention of bacteria colonization of implantable medical devices constitutes a major medical and financial issue. In this study, we developed an antibacterial coating based on self-assembled Fmoc-tripeptide. Fmoc-FFpY peptides (F: phenylalanine; Y: tyrosine; p: PO42-) are dephosphorylated enzymatically into Fmoc-FFY by action of alkaline phosphatase functionalized silica nanoparticles (NPs@AP), previously deposited on a surface. Fmoc-FFY peptides then self-assemble through pi-pi stacking interactions, hydrogen bonds and hydrophobic interactions adopting beta-sheets secondary structures. The obtained hydrogel coatings show fibrillary structures observed by cryo-scanning electron microscopy with a thickness of few micrometers. At low concentration (<= 0.5 mg.mL(-1)), self-assembled Fmoc-FFY has a superior antibacterial activity than Fmoc-FFpY peptide in solution. After 24 h of incubation, Fmoc-FFY hydrogel coatings fully inhibit the development of Gram-positiveStaphylococcus aureus (S. aureus). The antibacterial effect is maintained on anin vitromodel of repetitive infection in the case ofS. aureus. This coating could serve in infections were Gram positive bacteria are prevalent, e.g., intravascular catheter infections. This work gives new insights toward the design of an alternative antimicrobial coating.
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