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    Synthesis and characterisation of a mussel-inspired hydrogel film coating for biosensors

    Year: 2021

    Journal: Eur. Polym. J., Volume 153, JUN 15

    Authors: Millican, Jonathan M.; Bittrich, Eva; Caspari, Anja; Poschel, Kathrin; Drechsler, Astrid; Freudenberg, Uwe; Ryan, Timothy G.; Thompson, Richard L.; Pospiech, Doris; Hutchings, Lian R.

    Organizations: UK Engineering and Physical Sciences Research Council (EPSRC); Centre for Doctoral Training in Soft Matter and Functional Interfaces [EP/L015536/1]

    Keywords: Catechol; Copolymer; Hydrogel; Biosensor

    Mussel-inspired, catechol-containing monomers are being increasingly utilised to design versatile, adhesive, functional copolymers. This work reports the synthesis and detailed characterisation of a novel terpolymer comprising dopamine methacrylamide (DMA), in order to produce surface coatings capable of acting as biosensors for, e.g., toxins. DMA is copolymerised with 2-hydroxyethyl methacrylate (HEMA) and glycidyl methacrylate (GMA) using free radical polymerisation. The copolymers are subsequently deposited as a thin film using spin-coating. It is demonstrated that a terpolymer comprising HEMA, GMA and DMA can immobilise an IgG antibody on the film surface. The terpolymer therefore has the potential to be used as a coating in biosensing devices. It is also demonstrated that the presence of DMA impacts the copolymer properties. In-situ ellipsometry is used to confirm the important role of the catechol group on copolymer adhesion and significant desorption was observed in the absence of DMA.
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