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    The Impact of Mutations in the HvCPD and HvBRI1 Genes on the Physicochemical Properties of the Membranes from Barley Acclimated to Low/High Temperatures

    Year: 2020

    Journal: Cells, Volume 9, MAY

    Authors: Rudolphi-Szydlo, E; Sadura, I; Filek, M; Gruszka, D; Janeczko, A

    Organizations: National Science Centre (Poland)National Science Centre, Poland [2015/17/B/NZ9/01695]

    Keywords: brassinosteroids; cell membranes; Langmuir monolayers; galactolipids; plant acclimation to high; low temperature; phospholipids

    (1) Background: The study characterized barley mutants with brassinosteroid (BR) biosynthesis and signaling disturbances in terms of the physicochemical/structural properties of membranes to enrich the knowledge about the role of brassinosteroids for lipid metabolism and membrane functioning. (2) Methods: The Langmuir method was used to investigate the properties of the physicochemical membranes. Langmuir monolayers were formed from the lipid fractions isolated from the plants growing at 20 degrees C and then acclimated at 5 degrees C or 27 degrees C. The fatty acid composition of the lipids was estimated using gas chromatography. (3) Results: The BR-biosynthesis and BR-signaling mutants of barley were characterized by a temperature-dependent altered molar percentage of fatty acids (from 14:0 to 20:1) in their galactolipid and phospholipid fractions in comparison to wild-type (WT). For example, the mutants had a lower molar percentage of 18:3 in the phospholipid (PL) fraction. The same regularity was observed at 5 degrees C. It resulted in altered physicochemical parameters of the membranes (A(lim), pi (coll), Cs-1). (4) Conclusions: BR may be involved in regulating fatty acid biosynthesis or their transport/incorporation into the cell membranes. Mutants had altered physicochemical parameters of their membranes, compared to the WT, which suggests that BR may have a multidirectional impact on the membrane-dependent physiological processes.
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