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    Understanding the adsorption and potential tear film stability properties of recombinant human lubricin and bovine submaxillary mucins in an in vitro tear film model

    Year: 2020

    Journal: Colloid Surf. B-Biointerfaces, Volume 195, NOV

    Authors: Rabiah, Noelle, I; Sato, Yasunori; Kannan, Aadithya; Kress, Wolfgang; Straube, Frank; Fuller, Gerald G.

    Keywords: Lubricin; Mucins; Dry eye; Tear film; Fluid film stability; Wettability; Marangoni flow; QCM-D; Adsorption

    The wetting and adsorption properties for two glycoproteins, recombinant human lubricin and bovine submaxillary mucins (BSM) were evaluated on hydrophilic and hydrophobic glass dome surfaces in a simplified in vitro tear film model. We show that both recombinant human lubricin (rh-lubricin) and BSM solutions render surfaces hydrophilic and when the fluid films reach 500 nm or less, the fluids resist evaporation-driven breakup through a volumetric flux across the surface, which we believe is due to evaporation-driven solutocapillary flows. rh-Lubricin was able to maintain a wet film without spontaneous breakup for longer periods of time than BSM at lower concentrations, which we attribute to differences in adsorption properties, measured by QCM-D, that result from surface charge and structural differences (confirmed by zeta potential, DLS, and SAXS measurements).
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