Model membrane platforms are utilized in various fundamental and application research fields such as material science, bio-nanotechnology and drug discovery. To characterize these systems and understand their properties and behavior under various conditions, it is often necessary to use more than one analysis method. So which analysis methods should you use? To learn more, we talked to Prof. Nam-Joon Cho, Nanyang technological university in Singapore who has extensive experience working with lipid model systems. He shared with us a list of some of the technologies he uses in his research.
A mimic of the biological membrane
Lipid-based systems, monolayers, bilayers, and vesicles could be used as model membrane platforms. In particular, supported lipid bilayer systems have proven to be of special interest. Apart from the capability to mimic the biological membrane, supported lipid bilayer systems can be easily integrated with surface-based analytical techniques, which greatly facilitates the investigation of membrane related processes such as protein binding and cell-signaling.
Information extracted from complementary analytical methods can offer unique insights into the system under study
To characterize these systems and understand their properties and behavior under various conditions, it is often necessary to employ more than one analytical method. Information extracted from complementary analytical methods can offer unique insights into the system under investigation. Prof. Cho has extensive experience working with lipid model systems. Over the years, he has collected a set of technologies that he finds particularly useful in his lipid-related work. In Prof. Cho’s instrument portfolio, you will find technologies based on imaging as well as various quantification techniques.
Each of the analysis methods in his instrument portfolio provides key information about the system under study. Combined they enable him and his team to get a deeper understanding of properties and behavior of the lipid-based systems that they are studying.
In the overview, accessed below, Prof. Cho has compiled a list of analytical methods that he uses in his research to characterize lipid-based systems. The overview includes information about which technologies that he uses for his lipid-related work, what information each method offers, what are the pros and cons of the respective technology, and references to publications where the usage is exemplified.
Table 1. A brief excerpt from Prof. Cho's instrument portfolio overview, showing part of the information listed for one of the method that he uses.
Download the portfolio overview below to learn more about which technologies that could be used for lipid-based research, what the methods are suitable for and how they in combination can offer unique insights into the system under study.
Editor’s note: This post was originally published in July 2019 and has been updated for comprehensiveness
Malin graduated in engineering physics in 2006, where her research focused on the QCM-D technology. Since then, she has been scrutinizing the how’s and why’s of the world in general, and the world of QCM-D in particular.